ILE Extraction Well Plates
High-Throughput Extractions of Small Molecules Directly from Biological Fluids:
Immobilized Liquid Extraction™ (ILE) Well Plates are the perfect solution to your high-throughput extraction needs. ILE Well Plates provide analysts reliable, rapid and environmentally friendly high-throughput extractions without protein crashing, emulsion formation, solvent exchange or dry wells. ILE Well Plates involve minimal user management, especially on a well-to-well basis, do not require vacuum or pressure systems, and are capable of extracting small molecules directly from blood, serum or any aqueous biological matrix. Each well of an ILE Well Plate is coated with a thin layer of polymer (immobilized liquid) which acts as the medium of extraction. When a sample is directly exposed to the polymer, targeted analytes partition between the aqueous sample and the immobilized phase at a predictable rate. This partitioning process is allowed to occur until extraction equilibrium is attained. The quantity of a specific analyte that is extracted by the immobilized phase is determined both by the analyte’s relative affinity for both the sample and the immobilized phase (partition ratio), as well as by the relative volumes of the sample and the extracting polymer (phase ratio). The partitioning process may be accelerated by agitating the ILE Well Plate with a vortexer, sonicator or another appropriate method. Proper agitation disrupts a boundary layer which forms at the sample-polymer interface, a consequence which may alternatively be accomplished by repeatedly dispensing and aspirating a sample into and out of each well. Analytes which partition into the polymer are ‘back-extracted’, or desorped, into a small amount of LC or GC solvent, at which point the extraction is complete. New ILE Well Plate Applications! Example of Simple 3-Step Well Plate Procedure (1) Extract: Load samples into appropriate wells and thoroughly agitate (vortex, sonicate, etc.) ILE well-plate. Proper agitation ensures the disruption of a boundary layer which forms at the sample-polymer interface. This process is continued until reaction equilibrium is reached, or nearly reached and analytes have sufficiently partitioned from the sample into the immobilized phase.
ILE 96-Well Method Development Kit - Immobilized Liquid Extraction 96-Well extraction kits are a risk-free way to develop a method for your specific application. For $350, each kit includes three ILE Well Plates (PDMS, Acrylate, Fluoro)*, and full method development assistance. If you are unable to develop an appropriate method, simply return the plates to us within thirty days of receipt for a full refund. Or, if you decide to place an order for additional plates, the original purchase price of $350 will be credited toward your order.
More information about custom method development.
Polydimethylsiloxane (PDMS) - Relatively non-polar phase for extracting hydrophobic compounds. It has been experimentally shown that PDMS exhibits polarity and extractive properties similar to n-octanol. It is recommended as a general purpose ILE phase and is suitable for a wide range of extractions.
*Contact us for bulk pricing and standard order.
Polydiphenylsiloxane (Phenyl) - This is a more polar phase, and would be expected to display enhanced selectivity for aromatic compounds due to pi-bonding potential - a similar effect to extracting with toluene.
*Contact us for bulk pricing and standard order.
Trifluoropropylmethylsiloxane (Fluoro) - This phase provides enhanced selectivity for extracting compounds with lone pairs of electrons.
*Contact us for bulk pricing and standard order.
Polyacrylate (Acrylate) - Very polar phase which is used to extract hydrophilic and very polar compounds.
*Contact us for bulk pricing and standard order.
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