Coming Soon
A complete line of ILE pipette tips (patent pending) is currently in development. ILE pipette tips utilize the same polymeric (methyl, fluoro, and phenyl-polysiloxanes) coatings as other ILE products. The polymeric coating is immobilized on a portion of the inner surface of ILE pipette tips. ILE pipette tips extract compounds from aqueous matrices by repeatedly aspirating a sample into and out of the pipette tip until extraction equilibrium is attained. The analytes are next "back-extracted" into a small amount of solvent by aspirating enough of that solvent into the ILE pipette tip to cover the coated portion of the tip, and then expelled with the solvent as a sample ready for GC or LC analysis. No emulsions, sorbent conditioning, sorbent clogging, nor solvent waste.
ILE pipette tips allow an analyst to perform extractions both manually and through the use of automated liquid handling systems. Automated liquid handling systems fitted with ILE pipette tips are able to extract without the requirement of any special hardware such as vacuum systems and collection plates, as in SPE. Additionally, protein precipitation is not required. Derivitizing reagents do not affect the coating, so back extraction and derivitization can be performed simultaneously. Tips will be available in 20 µL and 200 µL versions in standard 96 well packs.
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Example of Simple 3-Step ILE Pipette Tip Procedure 
(1) Extract: Using an automated liquid handling system, samples are first aspirated into ILE the ILE Pipette Tips. Next, samples are repeatedly aspirated into the pipette tips and dispensed into a well plate, allowing repeated exposure of the sample to the immobilized solvent (polymer). This process is continued until reaction equilibrium is reached, or nearly reached, and analytes have sufficiently partitioned from the sample into the immobilized phase.
(2) Back-Extract: A small amount of method-appropriate solvent is aspirated into each ILE Pipette Tip from a second well plate -- a sufficient quantity of solvent will cover the entire coated portion of each ILE Pipette Tip. Compounds partition between the immobilized polymer and the solvent in accord with their relative affinities for each. This partitioning process may be accelerated by repeatedly aspirating and dispensing the solvent into and out of the pipette tip, and is allowed to occur until equilibrium is reached, or nearly reached.
(3) Analyze: The remaining solvent in each well may be immediately analyzed by GC or HPLC, or the well plate may be sealed and archived for later analysis.