Nitrogen/Phosphorous Pesticides from Whole Tomato

ILE Caps may be used to directly extract contaminants from fruits and vegetables. A tomato was placed in a 40mL VOA vial, chopped up to a fine consistency, spiked with a nitrogen/phosphorous pesticide mix, and diluted with water to a 1:1 ratio, by volume. 70µL Methylene Chloride was added to the diluted sample, and a 24mm ILE Cap was placed on the sample container. The sample vessel was agitated for one hour by a mechanical wrist shaker to facilitate compound partitioning into the immobilized solvent (polymer). Analytes were then back-extracted from the immobilized solvent (polymer) into 100µL acetone to complete the extraction process. The extract was analyzed in scan mode on an HP-5971 GC/MS with a 30m x 0.25mm i.d. x 0.25µm VB-5 column. The chromatogram is shown below.

Figure 1 (above): 1. Butylate, 2. Vernolate, 3. Pebulate, 4. Molinate, 5. Chlorpropham, 6. Prometon, 7. Atrazine, 8. Propazine, 9. Alachlor, 10. Metolachlor, 11. Triadimefon, 12. Diphenamid, 13. MGK-264.