Organohalide Pesticides from Orange Juice

Immobilized Liquid Extraction (ILE) Caps allow extractions from complex matrices without complications. An example of this is the extraction of organohalide pesticides from orange juice. A 20mL orange juice sample was placed in a 40mL VOA vial and spiked with 12µL of a 200µg/mL 505 organohalide pesticide mix. The sample vessel was sealed with a PDMS ILE Cap, inverted and agitated by a wrist shaker for one hour to allow adequate analyte partitioning from the sample into the immobilized phase. The ILE Cap was removed and placed on a high-recovery vial containing 200µL of methylene chloride. This vial was inverted to allow analytes to partition from the immobilized phase into the methylene chloride. The resulting extract was analyzed by an HP-5971 GC/MS in scan mode with a 30m x 0.25mm i.d. x 0.25µm VB-5 column. The chromatogram is shown below.

Figure 1 (above): 1. Methoxychlor, 2. Dieldrin, 3. Trans-Nonachlor, 4. Trans-Chlordane, 5. Endrin, 6. Chlordane, 7. Alachlor, 8. Heptachlor, 9. Limonene.